successful cryopreservation of in vitro derived bovine blastocysts in microcapillary pipette tips

نویسندگان

a. niasari-naslaji

p. j. hansen

k. moore

w. w. thatcher

چکیده

the open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. one possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. themain purpose of this study was to compare the survival rates of in vitro produced blastocysts followingvitrification in microcapillary pipette tips and open pulled straws. two experiments were conducted toinvestigate the effect of type of carrier, age of expanded blastocyst, and addition of β-mercaptoethanol topost-warming culture medium on survival of vitrified in-vitro derived blastocysts. expanded blastocysts (day7, 0900h and 1900h after insemination; insemination = day 0 at 0900h) were vitrified while loadedrandomly in groups of 4-10 into open pulled straws or pipette tips. following warming, embryos werecultured in groups of up to 20 in 20 μl microdrops of modified ksom or modified ksom containing 100μm β-mercaptoethanol at 38.5°c in 5% co2 in air. survival after warming was assessed as the percentage ofvitrified embryos that re-expanded and the percent that hatched after 48 hrs culture. post-warming survivalrates were not affected by type of carrier or age of the expanded blastocyst (p>0.05). the proportions ofembryos that re-expanded (55.5%) and hatched (25.7%) were higher (p

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Successful cryopreservation of in vitro derived bovine blastocysts in microcapillary pipette tips

The open pulled straw has been used effectively to vitrify preimplantation embryos because of geometricfeatures that allow rapid rates of temperature exchange. One possible inexpensive alternative to the openpulled straw are commercially-available microcapillary pipette tips commonly used for electrophoresis. Themain purpose of this study was to compare the survival rates of in vitro produced b...

متن کامل

P-83: Cloned Sheep Blastocysts Derived from Oocytes Enucleated Manually Using A Pulled Pasteur Pipette

Background: The potential applications of a simplified method of somatic cell nuclear transfer (SCNT) that is improved in both efficiency and throughput is considerable. Technically, a major step of SCNT is to produce large pools of enucleated oocytes (cytoplasts) efficiently, a process that requires considerable micromanipulation skill and expensive equipment. Here, we have devel oped an efficient...

متن کامل

Embryonic Stem-like Cells Derived from in vitro Produced Bovine Blastocysts

The aim of this work was to study the derivation of bovine embryonic stem-like (ES-like) cells from the inner cell mass (ICM) of in vitro produced blastocysts. The ICMs were mechanically isolated and six out of seventeen (35%) ICMs could attach to a monolayer of murine embryonic fibroblasts (MEF).Ten days after, primary outgrowths were mechanically dissected into several small clumps and transf...

متن کامل

Effect of the bovine oviductal fluid on in vitro fertilization, development and gene expression of in vitro-produced bovine blastocysts.

Oviductal microenvironment generally provides better conditions for early embryo development than the conventional in vitro system. In an attempt to simulate the oviduct conditions or the main potentially influencing factors, the effect was studied of a bovine oviductal fluid (bOF) treatment applied prior to IVF on (i) IVF parameters, (ii) cleavage rate, (iii) blastocyst yield and (iv) blastocy...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 8

شماره 1 2007

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023